Recombinant mouse GRP58/PDIA3 protein | NKMAX ATGP3620 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse GRP58/PDIA3 protein

catalog :

ATGP3620

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse GRP58/PDIA3 protein

Domain :

25-505aa

Catalog number (SKU) :

ATGP3620

NCBI :

NP_031978

Description :

PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant mouse PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Protein disulfide isomerase associated 3, 58 kDa glucose-regulated protein, GRP58, 58 kDa microsomal protein, p58, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, ER protein 57, ERp57, Endoplasmic reticulum resident protein 60, ER protein 60, ERp60, Erp, PDI, PDI-Q2, Plca

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, and 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

GilquinVigneron N., et al. (2009) Eur J Immunol. 39(9):2371-6. ;; Forster ML., et al. (2009) J Biol Chem. 284(19):13045-56

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >10 A650/cm/min/mg,obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Heat Shock Proteins

Molecular Weight :

56.8 kDa (505aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is >10 A650/cm/min/mg,obtained by measuring the increase of insulin precipitation in absorbance at 650nm resulting from the reduction of insulin.

Amino Acid Sequence :

MSDVLEL TDENFESRVS DTGSAGLMLV EFFAPWCGHC KRLAPEYEAA ATRLKGIVPL AKVDCTANTN TCNKYGVSGY PTLKIFRDGE EAGAYDGPRT ADGIVSHLKK QAGPASVPLR TEEEFKKFIS DKDASVVGFF RDLFSDGHSE FLKAASNLRD NYRFAHTNIE SLVKEYDDNG EGITIFRPLH LANKFEDKTV AYTEKKMTSG KIKKFIQDSI FGLCPHMTED NKDLIQGKDL LTAYYDVDYE KNAKGSNYWR NRVMMVAKKF LDAGHKLNFA VASRKTFSHE LSDFGLESTT GEVPVVAIRT AKGEKFVMQE EFSRDGKALE QFLQEYFDGN LKRYLKSEPI PESNEGPVKV VVAENFDDIV NEEDKDVLIE FYAPWCGHCK NLEPKYKELG EKLSKDPNIV IAKMDATAND VPSPYEVKGF PTIYFSPANK KLTPKKYEGG RELNDFISYL QREATNPPII QEEKPKKKKK AQEDL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P27773

Internal Search Terms :

PDIA3, Protein disulfide-isomerase A3 precursor, 58kDa, Erp, ERp57, ERp60, ERp61, Grp58, PDI, PDI-Q2, PI-PLC, Plca, PLC[a],ATGP3620-10ug,ATGP3620-20ug,ATGP3620-50ug,ATGP3620-100ug,ATGP3620-250ug,ATGP3620-500ug,ATGP3620-1mg,ATGP3620-10,ATGP3620-20,ATGP3620-50,ATGP3620-100,ATGP3620-250,ATGP3620-500,ATGP3620-1

more info or order :

NKMAX product webpage