protein

Recombinant human c-kit Ligand/SCF protein

ATGP3619

5 ug

80 USD

Recombinant human c-kit Ligand/SCF protein

26-189aa

ATGP3619

NP_000890

Stem Cell Factor (SCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. SCF binds to and activates the SCF receptor (SCFR), a receptor tyrosine kinase. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell-cell adhesion and acts synergistically with other cytokine. Recombinant human SCF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Kit ligand, KITLG, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, steel factor, SF, Kitl, KL-1, Familial progressive hyperpigmentation 2, FPH2, SLF, DFNA69

1mg/ml (determined by Bradford assay)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Zhang Z., et al. (2000). Proc. Natl. Acad. Sci. U.S.A. 97, 7732.;; Okada S , et al. (1992). Nippon Rinsho , 50, 1872.

> 95% by SDS-PAGE

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤10ng/ml.

SDS-PAGE, Bioactivity

Human

Adhesion & Movement

18.5 kDa (165aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤10 ng/ml.

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Non-Tagged

E.coli

Recombinant Protein

P21583

SCF,ATGP3619-10ug,ATGP3619-20ug,ATGP3619-50ug,ATGP3619-100ug,ATGP3619-250ug,ATGP3619-500ug,ATGP3619-1mg,ATGP3619-10,ATGP3619-20,ATGP3619-50,ATGP3619-100,ATGP3619-250,ATGP3619-500,ATGP3619-1

Lee SH, et al. Involvement of MITF-A, an alternative isoform of mi transcription factor, on the expression of tryptase gene in human mast cells. (Exp Mol Med. 2010){https://pubmed.ncbi.nlm.nih.gov/20513998/};;Takahashi M, et al. CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells. (Leukemia. 2014){https://pubmed.ncbi.nlm.nih.gov/24189293/};;Yamada T, et al. CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration. (Arch Dermatol Res. 2019){https://pubmed.ncbi.nlm.nih.gov/30483878/}