Recombinant human CD28 protein | NKMAX ATGP3603 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CD28 protein

catalog :

ATGP3603

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CD28 protein

Domain :

19-152aa

Catalog number (SKU) :

ATGP3603

NCBI :

NP_006130.1

Description :

CD28, also known as T-cell-specific surface glycoprotein CD28 isoform 1, is one of the proteins expressed on T cells that provide co-stimulatory signals required for T cell activation and survival. T cell stimulation through CD28 in addition to the T-cell receptor (TCR) can provide a potent signal for the production of various interleukins (IL-6 in particular). CD28/B7 interaction has been shown to prevent apoptosis of activated T cells via the up-regulation of bcl-XL. It ligation has also been shown to regulate Th1/Th2 differentiation. Recombinant human CD28, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

T-cell-specific surface glycoprotein CD28 isoform 1, CD28, Tp44

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Prasad KV., et al. (1994) National Academy. 91: 2834 -2838.;; Okkenhaug K., et al. (1998) J. Biol. Chem. 273: 21194 -21202.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human CD80 (CAT# ATGP3663). The ED50 range ≤ 60ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

42.4 kDa (376aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human CD80 (CAT# ATGP3663) is coated at 1 ug/ml (100 ul/well) can bind Human CD28. The ED50 range ≤ 60ug/ml.

Amino Acid Sequence :

NKILVKQ SPMLVAYDNA VNLSCKYSYN LFSREFRASL HKGLDSAVEV CVVYGNYSQQ LQVYSKTGFN CDGKLGNESV TFYLQNLYVN QTDIYFCKIE VMYPPPYLDN EKSNGTIIHV KGKHLCPSPL FPGPSKP

Tag :

hIgG-His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P10747

Internal Search Terms :

CD28,ATGP3603-10ug,ATGP3603-20ug,ATGP3603-50ug,ATGP3603-100ug,ATGP3603-250ug,ATGP3603-500ug,ATGP3603-1mg,ATGP3603-10,ATGP3603-20,ATGP3603-50,ATGP3603-100,ATGP3603-250,ATGP3603-500,ATGP3603-1

more info or order :

NKMAX product webpage