Recombinant renilla reniformis Luciferase protein | NKMAX ATGP3581 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant renilla reniformis Luciferase protein

catalog :

ATGP3581

quantity :

10 ug

price :

66 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant renilla reniformis Luciferase protein

Domain :

1-311aa

Catalog number (SKU) :

ATGP3581

NCBI :

AAA29804.1

Description :

LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. Recombinant renilla reniformis LUC, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Luciferase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Lorenz WW., et al. (1991) Proc Natl Acad Sci U S A. 88(10):4438-42.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >1X10^9 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at pH7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Renilla reniformis

Gene Category :

Enzymes & Proteases

Molecular Weight :

38.5 kDa (335aa) Confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is >1x10^9 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH7.5 at 25C.

Amino Acid Sequence :

MTSKVY DPEQRKRMIT GPQWWARCKQ MNVLDSFINY YDSEKHAENA VIFLHGNAAS SYLWRHVVPH IEPVARCIIP DLIGMGKSGK SGNGSYRLLD HYKYLTAWFE LLNLPKKIIF VGHDWGACLA FHYSYEHQDK IKAIVHAESV VDVIESWDEW PDIEEDIALI KSEEGEKMVL ENNFFVETML PSKIMRKLEP EEFAAYLEPF KEKGEVRRPT LSWPREIPLV KGGKPDVVQI VRNYNAYLRA SDDLPKMFIE SDPGFFSNAI VEGAKKFPNT EFVKVKGLHF SQEDAPDEMG KYIKSFVERV LKNEQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P27652

Internal Search Terms :

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more info or order :

NKMAX product webpage