Recombinant E.coli Beta-lactamase protein | NKMAX ATGP3575 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli Beta-lactamase protein

catalog :

ATGP3575

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli Beta-lactamase protein

Domain :

20-377aa

Catalog number (SKU) :

ATGP3575

NCBI :

NP_418574

Description :

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

ampC, ampA, Cephalosporinase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhao Z., et al. (2015) Cancer Lett. 358(1):17-26.;; Martin I., et al. (2014) Cell. 157(2):472-85.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Metabolism

Molecular Weight :

41.8 kDa (379aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

Amino Acid Sequence :

APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00811

Internal Search Terms :

b-lactamase,ATGP3575-10ug,ATGP3575-20ug,ATGP3575-50ug,ATGP3575-100ug,ATGP3575-250ug,ATGP3575-500ug,ATGP3575-1mg,ATGP3575-10,ATGP3575-20,ATGP3575-50,ATGP3575-100,ATGP3575-250,ATGP3575-500,ATGP3575-1

more info or order :

NKMAX product webpage