Recombinant mouse Cystatin C protein | NKMAX ATGP3569 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Cystatin C protein

catalog :

ATGP3569

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Cystatin C protein

Domain :

21-140aa

Catalog number (SKU) :

ATGP3569

NCBI :

NP_034106

Description :

CST3, also known as Cystatin-C is a member of family 2 of the cystatin superfamily. This protein is a secreted type 2 cysteine protease inhibitor synthesized in all nucleated cells, has been proposed as a replacement for serum creatinine for the assessment of renal function, particularly to detect small reductions in glomerular filtration rate. It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S. It indicates that a murine model should be relevant for studies of the human disease, hereditary Cystatin C amyloid angiopathy. Recombinant Mouse CST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

CST3, CysC

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Janowski. R., et al. (2001) Nat. Struct. Biol. 8(4):316-20.;; Mussap M., et al. (2004) Crit Rev Clin Lab Sci. 41(5-6):467-550.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Signal Transduction

Molecular Weight :

14.2 kDa (126aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

The IC50 value is < 1.0nM. The inhibitory function of Cystatin 3 on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C

Amino Acid Sequence :

ATPKQGPRML GAPEEADANE EGVRRALDFA VSEYNKGSND AYHSRAIQVV RARKQLVAGV NYFLDVEMGR TTCTKSQTNL TDCPFHDQPH LMRKALCSFQ IYSVPWKGTH SLTKFSCKNA

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala21

Product Type Name :

Recombinant Protein

UniProt No. :

P21460

Internal Search Terms :

Cystatin C, CST3, CysC,ATGP3569-10ug,ATGP3569-20ug,ATGP3569-50ug,ATGP3569-100ug,ATGP3569-250ug,ATGP3569-500ug,ATGP3569-1mg,ATGP3569-10,ATGP3569-20,ATGP3569-50,ATGP3569-100,ATGP3569-250,ATGP3569-500,ATGP3569-1

more info or order :

NKMAX product webpage