Recombinant human ECHS1 protein | NKMAX ATGP3567 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human ECHS1 protein

catalog :

ATGP3567

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human ECHS1 protein

Domain :

28-290aa

Catalog number (SKU) :

ATGP3567

NCBI :

AAH08906

Description :

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13.;; Stern J R., et al. (1956) J Biol Chem. 218:985-1002.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 150unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

30.6 kDa (284aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P30084

Internal Search Terms :

ECHS1, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH,ATGP3567-10ug,ATGP3567-20ug,ATGP3567-50ug,ATGP3567-100ug,ATGP3567-250ug,ATGP3567-500ug,ATGP3567-1mg,ATGP3567-10,ATGP3567-20,ATGP3567-50,ATGP3567-100,ATGP3567-250,ATGP3567-500,ATGP3567-1

more info or order :

NKMAX product webpage