Recombinant mouse Pin1 protein | NKMAX ATGP3566 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Pin1 protein

catalog :

ATGP3566

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Pin1 protein

Domain :

1-165aa

Catalog number (SKU) :

ATGP3566

NCBI :

NP_075860

Description :

Pin1, also known as peptidyl-prolyl cis-trans isomerase NIMA-interacting1, is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA, PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. Recombinant mouse PIN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Peptidyl-prolyl cis-trans isomerase NIMA-interacting1, 0610025L01Rik, D9Bwg1161e

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Wulf GM., et al. (2002) J Biol Chem. 277(50):47976-9;; Zacchi P., et al. (2002) Nature. 419(6909): 853-7.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,200nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Cell Cycle

Molecular Weight :

20.8 kDa (188aa) Confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 1,200 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSMADEEKL PPGWEKRMSR SSGRVYYFNH ITNASQWERP SGGSTVGGSS KNGQGEPAKV RCSHLLVKHS QSRRPSSWRQ EKITRSKEEA LELINGYIQK IKSGEEDFES LASQFSDCSS AKARGDLGPF SRGQMQKPFE DASFALRTGE MSGPVFTDSG IHIILRTE

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9QUR7

Internal Search Terms :

Pin1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting1, 0610025L01Rik, D9Bwg1161e,ATGP3566-10ug,ATGP3566-20ug,ATGP3566-50ug,ATGP3566-100ug,ATGP3566-250ug,ATGP3566-500ug,ATGP3566-1mg,ATGP3566-10,ATGP3566-20,ATGP3566-50,ATGP3566-100,ATGP3566-250,ATGP3566-500,ATGP3566-1

more info or order :

NKMAX product webpage