Recombinant human M-CSF protein | NKMAX ATGP3565 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human M-CSF protein

catalog :

ATGP3565

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human M-CSF protein

Domain :

33-255aa

Catalog number (SKU) :

ATGP3565

NCBI :

NP_000748

Description :

CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity. Recombinant human CSF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Macrophage colony-stimulating factor 1, CSF1, M-CSF, MCSF, Lanimostim, ATGP3269

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Chitu V., et al. (2006) Curr Opin Immunol. 18(1):39-48.;; Eda H., et al. (2010) Cytokine. 52(3):215-220.;; Tian F., et al. (2013) Oncol. Res. 20(8):341-350.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤3ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Additional Comment Only :

ATGP3269 has been replaced with a catalog number ATGP3565.

Molecular Weight :

26.1 kDa (231aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤3 ng/ml.

Amino Acid Sequence :

EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPR

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Glu33

Product Type Name :

Recombinant Protein

UniProt No. :

P09603

Internal Search Terms :

M-CSF,ATGP3565-10ug,ATGP3565-20ug,ATGP3565-50ug,ATGP3565-100ug,ATGP3565-250ug,ATGP3565-500ug,ATGP3565-1mg,ATGP3565-10,ATGP3565-20,ATGP3565-50,ATGP3565-100,ATGP3565-250,ATGP3565-500,ATGP3565-1

more info or order :

NKMAX product webpage