protein

Recombinant human PGP protein

ATGP3564

4 ug

30 USD

Recombinant human PGP protein

1-321aa

ATGP3564

NP_001035830

Phosphoglycolate phosphatase, also known as PGP, is detected in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. The highest activities occur in skeletal muscle and cardiac muscle. Recombinant human PGP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphoglycolate phosphatase

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Barker R.F., et al. (1978) Ann. Hum. Genet. 42:143-151

> 95% by SDS-PAGE

Specific activity is > 3,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Enzyme Activity,SDS-PAGE

Human

Metabolism

36.5 kDa (345aa) confirmed by MALDI-TOF

Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG

His-Tag

E.coli

Recombinant Protein

A6NDG6

PGP, Phosphoglycolate phosphatase,ATGP3564-10ug,ATGP3564-20ug,ATGP3564-50ug,ATGP3564-100ug,ATGP3564-250ug,ATGP3564-500ug,ATGP3564-1mg,ATGP3564-10,ATGP3564-20,ATGP3564-50,ATGP3564-100,ATGP3564-250,ATGP3564-500,ATGP3564-1