Recombinant human DUSP18 protein | NKMAX ATGP3562 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human DUSP18 protein

catalog :

ATGP3562

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human DUSP18 protein

Domain :

1-188aa

Catalog number (SKU) :

ATGP3562

NCBI :

NP_689724

Description :

Dual specificity phosphatase 18, also known as DUSP18, is a member of the dual-specificity phosphatase (DSP) family, which catalyzes dephosphorylation of phosphotyrosine and phosphothreonine residues. DUSP18 is inhibited by iodoarectic acid and is activated by manganese ions. Along with having preferential enzymatic activity against phosphorylated tyrosine residues over threonine residues, DUSP18 also dephosphorylates p-nitrophenyl phosphate (pNPP) in vitro. This protein is widely expressed with highest levels in liver, brain, ovary and testis. Recombinant human DUSP18 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Dual specificity protein phosphatase 18, DSP18, DUSP20, Low molecular weight dual specificity phosphatase 20, LMW-DSP20, LMWDSP20

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1mM PMSF, 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Jeong D G., et al. (2006) Acta Crystallogr. 62:582-588.;; Aoki N., et al. (2001) J Biochem. 130:133-140.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

23.6 kDa (212aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 300 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMTAPSC AFPVQFRQPS VSGLSQITKS LYISNGVAAN NKLMLSSNQI TMVINVSVEV VNTLYEDIQY MQVPVADSPN SRLCDFFDPI ADHIHSVEMK QGRTLLHCAA GVSRSAALCL AYLMKYHAMS LLDAHTWTKS CRPIIRPNSG FWEQLIHYEF QLFGKNTVHM VSSPVGMIPD IYEKEVRLMI PL

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q8NEJ0

Internal Search Terms :

DUSP18, Dual specificity protein phosphatase 18, DSP18, DUSP20,ATGP3562-10ug,ATGP3562-20ug,ATGP3562-50ug,ATGP3562-100ug,ATGP3562-250ug,ATGP3562-500ug,ATGP3562-1mg,ATGP3562-10,ATGP3562-20,ATGP3562-50,ATGP3562-100,ATGP3562-250,ATGP3562-500,ATGP3562-1

more info or order :

NKMAX product webpage