Recombinant human PHPT1 protein | NKMAX ATGP3560 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PHPT1 protein

catalog :

ATGP3560

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PHPT1 protein

Domain :

1-125aa

Catalog number (SKU) :

ATGP3560

NCBI :

NP_054891

Description :

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105(38):14442-6.;; van Vlierberghe P., et al. (2006) Leukemia. 20(7):1245-53.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >120unit/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

15.9 kDa (145aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9NRX4

Internal Search Terms :

PHPT1, 14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10,ATGP3560-10ug,ATGP3560-20ug,ATGP3560-50ug,ATGP3560-100ug,ATGP3560-250ug,ATGP3560-500ug,ATGP3560-1mg,ATGP3560-10,ATGP3560-20,ATGP3560-50,ATGP3560-100,ATGP3560-250,ATGP3560-500,ATGP3560-1

more info or order :

NKMAX product webpage