Recombinant human LIF protein | NKMAX ATGP3533 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human LIF protein

catalog :

ATGP3533

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human LIF protein

Domain :

23-202aa

Catalog number (SKU) :

ATGP3533

NCBI :

NP_002300

Description :

LIF, as known as leukemia inhibitory factor, is a pleiotropic glycoprotein belonging to the LI6 family of cytokines. This protein is involved in growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, embryogenesis and inflammation. It is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone. Also, it can function as an autocrine growth factor in some pancreatic cancers, but induced differentiation in the leukemic cell line M1. Recombinant human LIF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Leukemia inhibitory factor isoform1, LIF, CDF, DIA, HILDA, MLPLI

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kuphal S., et al, (2013) Exp. Mol. Pathol. 95:156-165.;; Liu SC., et al, (2013) J. Clin. Invest. 123:5269-5283.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 0.5 ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

20.8 kDa (189aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human LIF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 0.5ng/ml.

Amino Acid Sequence :

SPLPITP VNATCAIRHP CHNNLMNQIR SQLAQLNGSA NALFILYYTA QGEPFPNNLD KLCGPNVTDF PPFHANGTEK AKLVELYRIV VYLGTSLGNI TRDQKILNPS ALSLHSKLNA TADILRGLLS NVLCRLCSKY HVGHVDVTYG PDTSGKDVFQ KKKLGCQLLG KYKQIIAVLA QAF

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P15018

Internal Search Terms :

LIF, Leukemia inhibitory factor isoform1, LIF, CDF, DIA, HILDA, MLPLI,ATGP3533-10ug,ATGP3533-20ug,ATGP3533-50ug,ATGP3533-100ug,ATGP3533-250ug,ATGP3533-500ug,ATGP3533-1mg,ATGP3533-10,ATGP3533-20,ATGP3533-50,ATGP3533-100,ATGP3533-250,ATGP3533-500,ATGP3533-1

more info or order :

NKMAX product webpage