Recombinant rat Cyclophilin F/PPIF protein | NKMAX ATGP3496 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant rat Cyclophilin F/PPIF protein

catalog :

ATGP3496

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant rat Cyclophilin F/PPIF protein

Domain :

30-206aa

Catalog number (SKU) :

ATGP3496

NCBI :

NP_758443

Description :

Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CYPD, Cyclophilin F, Rotamase F

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Connern C.P., et al. (1992) Biochem. J. 284:381-385.;; Lin DT., et al. (2002) J Biol Chem. 23:277(34):31134-41.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,300nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Rat

Gene Category :

Enzymes & Proteases

Molecular Weight :

21.2Da (200aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P29117

Internal Search Terms :

Cyclophilin D, Cyclophilin F, CYP D, CYPD, CYP-D, mitochondrial, Peptidyl-prolyl cis-trans isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase, Ppif, ATGP3496-10ug, ATGP3496-20ug, ATGP3496-50ug, ATGP3496-100ug, ATGP3496-250ug, ATGP3496-500ug, ATGP3496-1mg, ATGP3496-010, ATGP3496-020, ATGP3496-050, ATGP3496-100, ATGP3496-250, ATGP3496-500, ATGP3496-01M

more info or order :

NKMAX product webpage