Recombinant human Beta-glucuronidase/GUSB protein | NKMAX ATGP3495 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Beta-glucuronidase/GUSB protein

catalog :

ATGP3495

quantity :

10 ug

price :

160 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Beta-glucuronidase/GUSB protein

Domain :

23-651aa

Catalog number (SKU) :

ATGP3495

NCBI :

NP_000172.2

Description :

GUSB, also known as beta-glucuronidase isoform 1, is a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans. It includes heparin sulfate, chondroitin sulfate and hyaluronan. Mutations in the GUSB are linked to mucopolysaccharidosis type VII. GUSB plays an important role in the degradation of dermatan and keratin sulfates. Recombinant human GUSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Beta-glucuronidase isoform 1, GUSB, BG, MPS7, Glucuronidase beta, Beta-G1

Concentration :

0.25mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Shipley JM., et al. (1993) Am J Hum Genet. 52:517-526.;; Bell CE Jr., et al. (1977) J Clin Invest. 59:97-105.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 4000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH3.5

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Signal Transduction

Molecular Weight :

73.4 kDa (635aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 1600 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-Methylumbelliferone to 4-Methylum-belliferyl-beta-D-glucosiduronic acid per minute at 37C and pH3.5

Amino Acid Sequence :

LQGGMLYPQE SPSRECKELD GLWSFRADFS DNRRRGFEEQ WYRRPLWESG PTVDMPVPSS FNDISQDWRL RHFVGWVWYE REVILPERWT QDLRTRVVLR IGSAHSYAIV WVNGVDTLEH EGGYLPFEAD ISNLVQVGPL PSRLRITIAI NNTLTPTTLP PGTIQYLTDT SKYPKGYFVQ NTYFDFFNYA GLQRSVLLYT TPTTYIDDIT VTTSVEQDSG LVNYQISVKG SNLFKLEVRL LDAENKVVAN GTGTQGQLKV PGVSLWWPYL MHERPAYLYS LEVQLTAQTS LGPVSDFYTL PVGIRTVAVT KSQFLINGKP FYFHGVNKHE DADIRGKGFD WPLLVKDFNL LRWLGANAFR TSHYPYAEEV MQMCDRYGIV VIDECPGVGL ALPQFFNNVS LHHHMQVMEE VVRRDKNHPA VVMWSVANEP ASHLESAGYY LKMVIAHTKS LDPSRPVTFV SNSNYAADKG APYVDVICLN SYYSWYHDYG HLELIQLQLA TQFENWYKKY QKPIIQSEYG AETIAGFHQD PPLMFTEEYQ KSLLEQYHLG LDQKRRKYVV GELIWNFADF MTEQSPTRVL GNKKGIFTRQ RQPKSAAFLL RERYWKIANE TRYPHSVAKS QCLENSLFT

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Leu23

Product Type Name :

Recombinant Protein

UniProt No. :

P08236

Internal Search Terms :

GUSB, Beta-glucuronidase isoform 1, GUSB, BG, MPS7,ATGP3495-10ug,ATGP3495-20ug,ATGP3495-50ug,ATGP3495-100ug,ATGP3495-250ug,ATGP3495-500ug,ATGP3495-1mg,ATGP3495-10,ATGP3495-20,ATGP3495-50,ATGP3495-100,ATGP3495-250,ATGP3495-500,ATGP3495-1

more info or order :

NKMAX product webpage