Recombinant human EphB2 protein | NKMAX ATGP3493 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human EphB2 protein

catalog :

ATGP3493

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human EphB2 protein

Domain :

19-543aa

Catalog number (SKU) :

ATGP3493

NCBI :

NP_059145

Description :

EPHB2, also known as ephrin type-B receptor 2 isoform 1, is a member of the transmembrane Eph receptor tyrosine kinase family (RTKs) that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin. Hippocampal neurons can release vesicles containing full length EPHB2, and these are taken up by neighboring glial cells. This protein controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity. Recombinant human EPHB2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

EPH receptor B2, Ephrin type-B receptor 2, EPHB2, Developmentally-regulated Eph-related tyrosine kinase, ELK-related tyrosine kinase, EPH tyrosine kinase 3, EPH-like kinase 5, EK5, hEK5, Renal carcinoma antigen NY-REN-47, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3, DRT, EPHT3, EPTH3, ERK, HEK5, TYRO5

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Pasquale EB. (2008) Cell. 133:38-52.;; Cowan CA., et al. (2000) Neuron 26:417-430.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured by its binding ability in a functional ELISA with Human Ephrin-B1 (CAT# ATGP3800)

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Cardiovascular

Molecular Weight :

59.1 kDa (533aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human EphB2 is coated at 2 ug/ml (100 ul/well) can bind Human Ephrin-B1 (CAT# ATGP3800) in a Functional ELISA assay.

Amino Acid Sequence :

VEETLMDSTT ATAELGWMVH PPSGWEEVSG YDENMNTIRT YQVCNVFESS QNNWLRTKFI RRRGAHRIHV EMKFSVRDCS SIPSVPGSCK ETFNLYYYEA DFDSATKTFP NWMENPWVKV DTIAADESFS QVDLGGRVMK INTEVRSFGP VSRSGFYLAF QDYGGCMSLI AVRVFYRKCP RIIQNGAIFQ ETLSGAESTS LVAARGSCIA NAEEVDVPIK LYCNGDGEWL VPIGRCMCKA GFEAVENGTV CRGCPSGTFK ANQGDEACTH CPINSRTTSE GATNCVCRNG YYRADLDPLD MPCTTIPSAP QAVISSVNET SLMLEWTPPR DSGGREDLVY NIICKSCGSG RGACTRCGDN VQYAPRQLGL TEPRIYISDL LAHTQYTFEI QAVNGVTDQS PFSPQFASVN ITTNQAAPSA VSIMHQVSRT VDSITLSWSQ PDQPNGVILD YELQYYEKEL SEYNATAIKS PTNTVTVQGL KAGAIYVFQV RARTVAGYGR YSGKMYFQTM TEAEYQTSIQ EKLPL

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Val19

Product Type Name :

Recombinant Protein

UniProt No. :

P29323

Internal Search Terms :

EphB2, Ephrin type-B receptor 2 isoform 1, EPHB2, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5,ATGP3493-10ug,ATGP3493-20ug,ATGP3493-50ug,ATGP3493-100ug,ATGP3493-250ug,ATGP3493-500ug,ATGP3493-1mg,ATGP3493-10,ATGP3493-20,ATGP3493-50,ATGP3493-100,ATGP3493-250,ATGP3493-500,ATGP3493-1

more info or order :

NKMAX product webpage