protein

Recombinant human IDO protein

ATGP3488

10 ug

60 USD

Recombinant human IDO protein

1-403aa

ATGP3488

NP_002155

IDO1 is an indoleamine 2, 3-dioxygenase - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, and serotonin. It is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan. Recombinant human IDO1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Metz R., et al (2007). Nature Cancer Res. 67:7082-7087;; Yuasa H.J., et al (2007). J. Mol. Evol. 65:705-714

> 90% by SDS-PAGE

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25C.

Enzyme Activity,SDS-PAGE

Human

Metabolism

47.7 kDa (426aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of L-Tryptophan to N-formyl-L-kynurenine per minute at pH 6.5 at 25C.

MGSSHHHHHH SSGLVPRGSH MGSMAHAMEN SWTISKEYHI DEEVGFALPN PQENLPDFYN DWMFIAKHLP DLIESGQLRE RVEKLNMLSI DHLTDHKSQR LARLVLGCIT MAYVWGKGHG DVRKVLPRNI AVPYCQLSKK LELPPILVYA DCVLANWKKK DPNKPLTYEN MDVLFSFRDG DCSKGFFLVS LLVEIAAASA IKVIPTVFKA MQMQERDTLL KALLEIASCL EKALQVFHQI HDHVNPKAFF SVLRIYLSGW KGNPQLSDGL VYEGFWEDPK EFAGGSAGQS SVFQCFDVLL GIQQTAGGGH AAQFLQDMRR YMPPAHRNFL CSLESNPSVR EFVLSKGDAG LREAYDACVK ALVSLRSYHL QIVTKYILIP ASQQPKENKT SEDPSKLEAK GTGGTDLMNF LKTVRSTTEK SLLKEG

His-Tag

E.coli

Recombinant Protein

P14902

IDO, Indoleamine 2,3-dioxygenase 1, IDO, IDO-1, INDO,ATGP3488-10ug,ATGP3488-20ug,ATGP3488-50ug,ATGP3488-100ug,ATGP3488-250ug,ATGP3488-500ug,ATGP3488-1mg,ATGP3488-10,ATGP3488-20,ATGP3488-50,ATGP3488-100,ATGP3488-250,ATGP3488-500,ATGP3488-1