Recombinant human CNTF protein | NKMAX ATGP3455 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human CNTF protein

catalog :

ATGP3455

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human CNTF protein

Domain :

1-200aa

Catalog number (SKU) :

ATGP3455

NCBI :

NP_000605

Description :

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Ciliary neurotrophic factor

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sendther M., et al. (1994), J Neurobiol. 25(11):1436-53.;; Robert F., et al. (2007), J. Biol. Chem. 282(46): 33421-33434

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1ug/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

25 kDa (220aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human CNTF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1ug/ml.

Amino Acid Sequence :

MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P26441

Internal Search Terms :

CNTF, Ciliary neurotrophic factor,ATGP3455-10ug,ATGP3455-20ug,ATGP3455-50ug,ATGP3455-100ug,ATGP3455-250ug,ATGP3455-500ug,ATGP3455-1mg,ATGP3455-10,ATGP3455-20,ATGP3455-50,ATGP3455-100,ATGP3455-250,ATGP3455-500,ATGP3455-1

more info or order :

NKMAX product webpage