Recombinant mouse GAPDH protein | NKMAX ATGP3434 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse GAPDH protein

catalog :

ATGP3434

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse GAPDH protein

Domain :

1-333aa

Catalog number (SKU) :

ATGP3434

NCBI :

NP_032110

Description :

Gapdh, also known as glyceraldehyde 3-phosphate dehydrogenase, is an enzyme of 37kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In addition to this long established metabolic function, Gapdh has recently been implicated in several non-metabolic processes, including transcription activation, initiation of apoptosis, ER to Golgi vesicle shuttling, and fast axonal, or axoplasmic transport. Recombinant mouse Gapdh, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Glyceraldehyde-3-phosphate dehydrogenase isoform 2, G3PD, GAPD, HEL-S-162eP, Peptidyl-cysteine S-nitrosylase GAPDH

Concentration :

0.25mg/ml (determined by Bradford assay)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tarze A, Deniaud A, et al. (2007). Oncogene. 26(18):2606-20.;; Tisdale EJ, et al. (2007). Traffic. 8(6):733-41.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 40unit/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Enzymes & Proteases

Molecular Weight :

38.2 kDa (356aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 40 units/mg, and is defined as theamount of enzyme that convert 1.0 umole of glyceraldehyde-3-phosphate to 1,3-Bisphosphoglycerate per minute at pH 8.5 at 37C.

Amino Acid Sequence :

MVKVGVN GFGRIGRLVT RAAICSGKVE IVAINDPFID LNYMVYMFQY DSTHGKFNGT VKAENGKLVI NGKPITIFQE RDPTNIKWGE AGAEYVVEST GVFTTMEKAG AHLKGGAKRV IISAPSADAP MFVMGVNHEK YDNSLKIVSN ASCTTNCLAP LAKVIHDNFG IVEGLMTTVH AITATQKTVD GPSGKLWRDG RGAAQNIIPA STGAAKAVGK VIPELNGKLT GMAFRVPTPN VSVVDLTCRL EKPAKYDDIK KVVKQASEGP LKGILGYTED QVVSCDFNSN SHSSTFDAGA GIALNDNFVK LISWYDNEYG YSNRVVDLMA YMASKE

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P16858

Internal Search Terms :

GAPDH,ATGP3434-10ug,ATGP3434-20ug,ATGP3434-50ug,ATGP3434-100ug,ATGP3434-250ug,ATGP3434-500ug,ATGP3434-1mg,ATGP3434-10,ATGP3434-20,ATGP3434-50,ATGP3434-100,ATGP3434-250,ATGP3434-500,ATGP3434-1

more info or order :

NKMAX product webpage