protein

Recombinant human Guanylate kinase 1/GUK1 protein

ATGP3432

10 ug

80 USD

Recombinant human Guanylate kinase 1/GUK1 protein

1-197aa

ATGP3432

NP_000849

GUK1, also known as GMK, belongs to the guanylate kinase family. This protein exists as a monomer that catalyzes the ATP-dependent conversion of GMP to GDP, thereby playing an essential role in the recycling of GMP. Via its catalytic activity, GUK1 is thought to participate in regulating the supply of guanine nucleotides to signal transduction pathways. Overexpression of GUK1 is associated with pituitary adenocarcinomas, suggesting that GUK1 is involved in tumorigenesis. Recombinant human GUK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Guanylate kinase, GMK, GMP kinase, GMPK

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Da Rocha AA., et al. (2006) Pituitary. 9(2):83-92. ;; Brady WA., et al. (1996) J Biol Chem. 271(28):16734-40

> 90% by SDS-PAGE

Specific activity is >100unit/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

SDS-PAGE, Enzyme Activity

Human

Cancer

23.9 kDa (217aa) confirmed by MALDI-TOF

Specific activity is >100 units/mg and is defined as the amount of enzyme that convert 1.0 umole of GMP and ATP to GDP and ADP per minute at pH 7.5 at 37C in coupled system with PK/LDH.

MGSSHHHHHH SSGLVPRGSH MSGPRPVVLS GPSGAGKSTL LKRLLQEHSG IFGFSVSHTT RNPRPGEENG KDYYFVTREV MQRDIAAGDF IEHAEFSGNL YGTSKVAVQA VQAMNRICVL DVDLQGVRNI KATDLRPIYI SVQPPSLHVL EQRLRQRNTE TEESLVKRLA AAQADMESSK EPGLFDVVII NDSLDQAYAE LKEALSEEIK KAQRTGA

His-Tag

E.coli

Recombinant Protein

Q16774

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