Recombinant human Monoglyceride Lipase/MGLL protein | NKMAX ATGP3430 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Monoglyceride Lipase/MGLL protein

catalog :

ATGP3430

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Monoglyceride Lipase/MGLL protein

Domain :

1-313aa

Catalog number (SKU) :

ATGP3430

NCBI :

NP_009214

Description :

MGLL is membrane-associated member of the serine hydrolase superfamily. MGLL functions together with hormone-sensitive lipase (LIPE) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides. It is most abundantly expressed in skeletal muscle and adipose tissue. Recombinant human MGLL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Monoglyceride lipase, Hu-K5, HuK5, MAGL, MGL, Lysophospholipase homolog, Lysophospholipase-like, Monoacylglycerol lipase

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cravatt BF., et al. (2010) Cell. 140(1):49-61.;; Lambert DM., et al (2010) Chembiochem.11(2):218-27.

Purity :

> 85% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >170unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

36.4 kDa (333aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >170 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH 7.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH METGPEDPSS MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE LARMLMGLDL LVFAHDHVGH GQSEGERMVV SDFHVFVRDV LQHVDSMQKD YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV SQRTATAGTA SPP

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q99685

Internal Search Terms :

Hu K5, HuK5, Hu-K5, Lysophospholipase homolog, Lysophospholipase-like, MAGL, MGL, MGLL, Monoglyceride Lipase, ATGP3430-10ug, ATGP3430-20ug, ATGP3430-50ug, ATGP3430-100ug, ATGP3430-250ug, ATGP3430-500ug, ATGP3430-1mg, ATGP3430-010, ATGP3430-020, ATGP3430-050, ATGP3430-100, ATGP3430-250, ATGP3430-500, ATGP3430-01M

more info or order :

NKMAX product webpage