Recombinant human PGAM2 protein | NKMAX ATGP3428 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PGAM2 protein

catalog :

ATGP3428

quantity :

10 ug

price :

101 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PGAM2 protein

Domain :

1-253aa

Catalog number (SKU) :

ATGP3428

NCBI :

NP_000281

Description :

PGAM2, also known as phosphoglycerate mutase 2, belongs to the phosphoglycerate mutase family. Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Tsujino S., et al. (1989) J. Biol. Chem. 264:15334-15337;; Hadjigeorgiou G.M., et al. (1999) Neuromuscul. Disord. 9:399-402

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >100unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

30.9 kDa (273aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >100units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MATHRLVMVR HGESTWNQEN RFCGWFDAEL SEKGTEEAKR GAKAIKDAKM EFDICYTSVL KRAIRTLWAI LDGTDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEE QVKIWRRSFD IPPPPMDEKH PYYNSISKER RYAGLKPGEL PTCESLKDTI ARALPFWNEE IVPQIKAGKR VLIAAHGNSL RGIVKHLEGM SDQAIMELNL PTGIPIVYEL NKELKPTKPM QFLGDEETVR KAMEAVAAQG KAK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P15259

Internal Search Terms :

PGAM2, Phosphoglycerate mutase 2, GSD10, PGAM-M, PGAMM,ATGP3428-10ug,ATGP3428-20ug,ATGP3428-50ug,ATGP3428-100ug,ATGP3428-250ug,ATGP3428-500ug,ATGP3428-1mg,ATGP3428-10,ATGP3428-20,ATGP3428-50,ATGP3428-100,ATGP3428-250,ATGP3428-500,ATGP3428-1

more info or order :

NKMAX product webpage