Recombinant mouse PGAM1 protein | NKMAX ATGP3427 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse PGAM1 protein

catalog :

ATGP3427

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse PGAM1 protein

Domain :

1-254aa

Catalog number (SKU) :

ATGP3427

NCBI :

NP_075907

Description :

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Recombinant mouse PGAM1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Cao L., et al. (2007) J. Immunol. 179:5864-5876;; Ballif B.A., et al. (2008) J. Proteome Res. 7:311-318

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >150unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Mouse

Gene Category :

Cancer

Molecular Weight :

31.4 kDa (278aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >150units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MGSHMAAYKL VLIRHGESAW NLENRFSGWY DADLSPAGHE EAKRGGQALR DAGYEFDICF TSVQKRAIRT LWTVLDAIDQ MWLPVVRTWR LNERHYGGLT GLNKAETAAK HGEAQVKIWR RSYDVPPPPM EPDHPFYSNI SKDRRYADLT EDQLPSCESL KDTIARALPF WNEEIVPQIK EGKRVLIAAH GNSLRGIVKH LEGLSEEAIM ELNLPTGIPI VYELDKNLKP IKPMQFLGDE ETVRKAMEAV AAQGKVKK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9DBJ1

Internal Search Terms :

PGAM1, Phosphoglycerate mutase 1, 2310050F24Rik, Pgam-1,ATGP3427-10ug,ATGP3427-20ug,ATGP3427-50ug,ATGP3427-100ug,ATGP3427-250ug,ATGP3427-500ug,ATGP3427-1mg,ATGP3427-10,ATGP3427-20,ATGP3427-50,ATGP3427-100,ATGP3427-250,ATGP3427-500,ATGP3427-1

more info or order :

NKMAX product webpage