Recombinant human PGAM1 protein | NKMAX ATGP3426 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PGAM1 protein

catalog :

ATGP3426

quantity :

10 ug

price :

45 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PGAM1 protein

Domain :

1-254aa

Catalog number (SKU) :

ATGP3426

NCBI :

NP_002620.1

Description :

PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2, 3-BPGA (2, 3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Sakoda S., et al. (1988) J Biol Chem. 263(32):16899-905. ;; Junien C., et al. (1982) Ann Genet. 25(1):25-7.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is >300unit/mg, in which one unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

30.9 kDa (274aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37C.

Amino Acid Sequence :

MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P18669

Internal Search Terms :

PGAM1, Phosphoglycerate mutase 1, Phosphoglycerate mutase isozyme B, PGAM-B, PGAMA,ATGP3426-10ug,ATGP3426-20ug,ATGP3426-50ug,ATGP3426-100ug,ATGP3426-250ug,ATGP3426-500ug,ATGP3426-1mg,ATGP3426-10,ATGP3426-20,ATGP3426-50,ATGP3426-100,ATGP3426-250,ATGP3426-500,ATGP3426-1

more info or order :

NKMAX product webpage