Recombinant human LTA/TNF-beta protein | NKMAX ATGP3420 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human LTA/TNF-beta protein

catalog :

ATGP3420

quantity :

10 ug

price :

130 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human LTA/TNF-beta protein

Domain :

35-205aa

Catalog number (SKU) :

ATGP3420

NCBI :

NP_001153212

Description :

LTA, also known as lymphotoxin alpha and tumor necrosis factor beta, belongs to the tumor necrosis factor family. It is highly inducible, secreted, and forms heterotrimers with lymphotoxin beta which anchor lymphotoxin alpha to the cell surface. It is expressed by activated naive CD4 cells, unpolarized IL2 secreting effectors, and Th1 effectors and mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. Recombinant human LTA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Alternative Names :

Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, TNFB, TNFSF1, TNF superfamily, member 1

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Messer G., et al. (1991) J Exp Med. 173:209-219.;; Gramaglia I., et al. (1999) J Immunol 162:1333-1338

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 1ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

19.7 kDa (180aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human LTA/TNF-beta induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Amino Acid Sequence :

LPGVGLT PSAAQTARQH PKMHLAHSTL KPAAHLIGDP SKQNSLLWRA NTDRAFLQDG FSLSNNSLLV PTSGIYFVYS QVVFSGKAYS PKATSSPLYL AHEVQLFSSQ YPFHVPLLSS QKMVYPGLQE PWLHSMYHGA AFQLTQGDQL STHTDGIPHL VLSPSTVFFG AFAL

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Ala

Product Type Name :

Recombinant Protein

UniProt No. :

P01374

Internal Search Terms :

Lymphotoxin-a , TNF-b, Lymphotoxin alpha, LTA, LT, TNFB, TNFSF1, TNLG1E, LTalpha, DIF, LT, LT alpha, LT-alpha, Lta, Lymphotoxin alpha, Lymphotoxin alpha (TNF superfamily, member 1), Lymphotoxin-alpha, TNF B, TNF superfamily member 1, TNF-beta, TNFB, TNFB_HUMAN, TNFbeta, TNFSF1, Tumor necrosis factor beta, Tumor necrosis factor ligand superfamily member 1,ATGP3420-10ug,ATGP3420-20ug,ATGP3420-50ug,ATGP3420-100ug,ATGP3420-250ug,ATGP3420-500ug,ATGP3420-1mg,ATGP3420-10,ATGP3420-20,ATGP3420-50,ATGP3420-100,ATGP3420-250,ATGP3420-500,ATGP3420-1

more info or order :

NKMAX product webpage