Recombinant human Lactate Dehydrogenase B/LDHB protein | NKMAX ATGP3387 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Lactate Dehydrogenase B/LDHB protein

catalog :

ATGP3387

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Lactate Dehydrogenase B/LDHB protein

Domain :

1-334aa

Catalog number (SKU) :

ATGP3387

NCBI :

NP_002291

Description :

LDHB, also known as L-lactate dehydrogenase B chain isoform LDHB, is a member of the lactate dehydrogenase family. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As this protein can also catalyze the oxidation of hydroxybutyrate. The LDH family consists of three members, LDH-A, LDH-B and LDH-C. LDHs function as powerful markers for germ cell tumors. Recombinant human LDHB protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Renal carcinoma antigen NY-REN-46, Lactate dehydrogenase B, L-lactate dehydrogenase B chain, LDH-H, LDH-B, LDH heart subunit

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Zhao Z., et al. (2015) Cancer Lett. 358(1):17-26.;; Martin I., et al. (2014) Cell. 157(2):472-85.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

36.6 kDa (334aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 300 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MATLKEKLIA PVAEEEATVP NNKITVVGVG QVGMACAISI LGKSLADELA LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG LTSVINQKLK DDEVAQLKKS ADTLWDIQKD LKDL

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P07195

Internal Search Terms :

Lactate Dehydrogenase B, LDHB, Lactate dehydrogenase B, HEL-S-281, LDH-B, LDH-H, LDHBD, TRG-5, Epididymis secretory protein Li 281, HEL S 281, L lactate dehydrogenase B chain, L-lactate dehydrogenase B chain, Lactate dehydrogenase H chain, LDH B, LDH H, LDH heart subunit, LDH-B, LDH-H, LDHB, LDHB_HUMAN, LDHBD, LDHH, Renal carcinoma antigen NY REN 46, Renal carcinoma antigen NY-REN-46, TRG-5, TRG5,ATGP3387-10ug,ATGP3387-20ug,ATGP3387-50ug,ATGP3387-100ug,ATGP3387-250ug,ATGP3387-500ug,ATGP3387-1mg,ATGP3387-10,ATGP3387-20,ATGP3387-50,ATGP3387-100,ATGP3387-250,ATGP3387-500,ATGP3387-1

more info or order :

NKMAX product webpage