product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant rat Lactate Dehydrogenase A/LDHA protein
catalog :
ATGP3368
quantity :
10 ug
price :
105 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant rat Lactate Dehydrogenase A/LDHA protein
Domain :
1-332aa
Catalog number (SKU) :
ATGP3368
NCBI :
NP_058721
Description :
Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Alternative Names :
LDH muscle subunit, LDH-M
Concentration :
0.25mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Ward RA., et al. (2012) J Med Chem. 55:3285-3306.;; Fantin VR., et al. (2006) Cancer Cell. 9:425-434.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Rat
Gene Category :
Signal Transduction
Molecular Weight :
37.5 kDa (340aa)
Endotoxin :
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment and Pics :
Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
Amino Acid Sequence :
MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
Tag :
His-Tag
Expression System :
Baculovirus
Predicted nterm :
Met1
Product Type Name :
Recombinant Protein
UniProt No. :
P04642
Internal Search Terms :
Lactate Dehydrogenase A, LDHA, L-lactate dehydrogenase A chain, Ldha, Ldh1,ATGP3368-10ug,ATGP3368-20ug,ATGP3368-50ug,ATGP3368-100ug,ATGP3368-250ug,ATGP3368-500ug,ATGP3368-1mg,ATGP3368-10,ATGP3368-20,ATGP3368-50,ATGP3368-100,ATGP3368-250,ATGP3368-500,ATGP3368-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.