Recombinant mouse Glyoxalase I protein | NKMAX ATGP3342 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant mouse Glyoxalase I protein

catalog :

ATGP3342

quantity :

10 ug

price :

105 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant mouse Glyoxalase I protein

Domain :

1-184aa

Catalog number (SKU) :

ATGP3342

NCBI :

NP_079650

Description :

Glo1, also known as lactoylglutathione lyase, is a member of the glyoxalase l family. It plays a critical role in the detoxification of 2-oxoaldehydes, such as methylglyoxal. It involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. It was identified as a protein marker, which is consistently expressed to a higher extent in LAB-M than in HAB-M mice in several brain areas. Recombinant mouse Glo1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Glyoxalase 1, Lactoylglutathione lyase, 0610009E22Rik, 1110008E19Rik, 2510049H23Rik, AW550643, Glo-1, Glo-1r, Glo-1s, Glo1-r, Glo1-s, GLY1, Qglo

Concentration :

0.5mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Kawatani M., et al. (2008) Proc Natl Acad Sci U S A. 105:11691-11696.;; Kromer SA., et al. (2005) J Neurosci. 25:4375-4384.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 210unit/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Mouse

Gene Category :

Signal Transduction

Molecular Weight :

21.8 kDa (192aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 micromol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.

Amino Acid Sequence :

MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH

Tag :

His-Tag

Expression System :

Baculovirus

Predicted nterm :

Met1

Product Type Name :

Recombinant Protein

UniProt No. :

Q9CPU0

Internal Search Terms :

Glyoxalase I, Glyoxalase 1, Lactoylglutathione lyase, 0610009E22Rik, 1110008E19Rik, 2510049H23Rik, AW550643, Glo-1, Glo-1r, Glo-1s, Glo1-r, Glo1-s, GLY1, Qglo,ATGP3342-10ug,ATGP3342-20ug,ATGP3342-50ug,ATGP3342-100ug,ATGP3342-250ug,ATGP3342-500ug,ATGP3342-1mg,ATGP3342-10,ATGP3342-20,ATGP3342-50,ATGP3342-100,ATGP3342-250,ATGP3342-500,ATGP3342-1

more info or order :

NKMAX product webpage