protein

Recombinant human TPST1 protein

ATGP3331

4 ug

80 USD

Recombinant human TPST1 protein

26-370aa

ATGP3331

NP_003587

TPST1, also known as protein-tyrosine sulfotransferase 1, is the enzyme that catalyzes the sulfation reaction of protein tyrosines, a post-translational modification of proteins. It utilizes 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) as the sulfonate donor and binds proteins with target tyrosine residues to eventually form the tyrosine O-sulfate ester group and the desulfonated 3'-phosphoadenosine-5'-phosphate (PAP). Recombinant human TPST1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Protein-tyrosine sulfotransferase 1, TANGO13A

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Ouyang Y.B., et al. (1998) Proc Natl Acad Sci USA. 95:2896-2901.;; Danan L.M., et al. (2008) J. Am. Soc. Mass. Spectrom. 19:1459-1466.

> 90% by SDS-PAGE

SDS-PAGE

Human

Cardiovascular

40.6 kDa (354aa)

< 1 EU per 1ug of protein (determined by LAL method)

QHAMECH HRIEERSQPV KLESTRTTVR TGLDLKANKT FAYHKDMPLI FIGGVPRSGT TLMRAMLDAH PDIRCGEETR VIPRILALKQ MWSRSSKEKI RLDEAGVTDE VLDSAMQAFL LEIIVKHGEP APYLCNKDPF ALKSLTYLSR LFPNAKFLLM VRDGRASVHS MISRKVTIAG FDLNSYRDCL TKWNRAIETM YNQCMEVGYK KCMLVHYEQL VLHPERWMRT LLKFLQIPWN HSVLHHEEMI GKAGGVSLSK VERSTDQVIK PVNVGALSKW VGKIPPDVLQ DMAVIAPMLA KLGYDPYANP PNYGKPDPKI IENTRRVYKG EFQLPDFLKE KPQTEQVE

His-Tag

Baculovirus

Ala

Recombinant Protein

O60507

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