protein

Recombinant human PTH protein

ATGP3317

10 ug

80 USD

Recombinant human PTH protein

32-115aa

ATGP3317

NP_000306

Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques

PTH, PTH1

1mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM EDTA

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88(11), 5212-20.;; Wingender E., et al. (1989) J. Biol. Chem. 264(8),4367-73.

> 95% by SDS-PAGE

Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50ng/ml.

SDS-PAGE, Bioactivity

Human

Hormones & Cytokines

9.55 kDa (85aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Human PTH Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50 ng/ml.

MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ

Non-Tagged

E.coli

Recombinant Protein

P01270

PTH, PTH, PTH1,ATGP3317-10ug,ATGP3317-20ug,ATGP3317-50ug,ATGP3317-100ug,ATGP3317-250ug,ATGP3317-500ug,ATGP3317-1mg,ATGP3317-10,ATGP3317-20,ATGP3317-50,ATGP3317-100,ATGP3317-250,ATGP3317-500,ATGP3317-1

Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009){https://pubmed.ncbi.nlm.nih.gov/19576433/}