protein

Recombinant human SORD protein

ATGP3293

10 ug

80 USD

Recombinant human SORD protein

1-357aa

ATGP3293

NP_003095.1

SORD, also known as sorbitol dehydrogenase, is a member of the zinc-containing alcohol dehydrogenase family. It is widely expressed with highest expression in kidney and in the lens of the eye. SORD enzymatically catalyzes the zinc-dependent interconversion of polyols, such as sorbitol and xylitol, to their respective ketoses. Recombinant human SORD protein, was expressed in E. coli and purified by using conventional chromatography techniques.

SORD1, RDH, SDH, SORDD, XDH

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 10% glycerol, 1mM DTT

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Baker MA., et al. (2010) Proteomics. 10(3):482-95.;; Sorger D., et al. (2009) Nucl Med Biol. 36(1):17-27.

> 90% by SDS-PAGE

Specific activity is > 15unit/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

SDS-PAGE, Enzyme Activity

Human

Enzymes & Proteases

38.3 kDa (357aa)

Specific activity is > 15 units/mg, and is defined as the amount of enzyme that catalyze the reduction 1.0 umole of D-fructose to D-sorbitol per minute at pH 7.5 at 37C.

MAAAAKPNNL SLVVHGPGDL RLENYPIPEP GPNEVLLRMH SVGICGSDVH YWEYGRIGNF IVKKPMVLGH EASGTVEKVG SSVKHLKPGD RVAIEPGAPR ENDEFCKMGR YNLSPSIFFC ATPPDDGNLC RFYKHNAAFC YKLPDNVTFE EGALIEPLSV GIHACRRGGV TLGHKVLVCG AGPIGMVTLL VAKAMGAAQV VVTDLSATRL SKAKEIGADL VLQISKESPQ EIARKVEGQL GCKPEVTIEC TGAEASIQAG IYATRSGGTL VLVGLGSEMT TVPLLHAAIR EVDIKGVFRY CNTWPVAISM LASKSVNVKP LVTHRFPLEK ALEAFETFKK GLGLKIMLKC DPSDQNP

Non-Tagged

E.coli

Recombinant Protein

Q00796

SORD, SORD1,ATGP3293-10ug,ATGP3293-20ug,ATGP3293-50ug,ATGP3293-100ug,ATGP3293-250ug,ATGP3293-500ug,ATGP3293-1mg,ATGP3293-10,ATGP3293-20,ATGP3293-50,ATGP3293-100,ATGP3293-250,ATGP3293-500,ATGP3293-1