Recombinant human Aldo-keto Reductase 1C3/AKR1C3 protein

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Aldo-keto Reductase 1C3/AKR1C3 protein

catalog :

ATGP3276

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Aldo-keto Reductase 1C3/AKR1C3 protein

Domain :

1-323aa

Catalog number (SKU) :

ATGP3276

NCBI :

NP_003730.4

Description :

AKR1C3 also known as Aldo-keto reductase family 1 member C3 isoform 1, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation. Recombinant human AKR1C3 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Aldo-keto reductase family 1 member C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, Aldo-keto reductase family 1, member C3 17 beta HSD 5(HSD17B5), AKR1 C3, Chlordecone reductase, 17 beta hydroxysteroid dehydrogenase type 5, DDH1, PGFS, Prostaglandin F synthase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Davies NJ., et al. (2009) Cancer Res. 69(11):4769-75.;; Kabututu Z., et al. (2009) J Biochem. 145(2):161-8.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 1,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

36.8 kDa (323aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Amino Acid Sequence :

MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY

Tag :

Non-Tagged

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P42330

Internal Search Terms :

Aldo-keto Reductase1C3, AKR1C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, PGFS,ATGP3276-10ug,ATGP3276-20ug,ATGP3276-50ug,ATGP3276-100ug,ATGP3276-250ug,ATGP3276-500ug,ATGP3276-1mg,ATGP3276-10,ATGP3276-20,ATGP3276-50,ATGP3276-100,ATGP3276-250,ATGP3276-500,ATGP3276-1

more info or order :

NKMAX product webpage