protein

Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

ATGP3275

4 ug

30 USD

Recombinant human Aldo-keto Reductase 1C1/AKR1C1 protein

1-323aa

ATGP3275

NP_001344

AKR1C1 also known as Aldo-keto reductase family 1 member C1, is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant human AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.5) containing 0.1M NaCl, 20% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25(2):221-8.;; Zhang Y., et al. (2009) Mol Cell Endicrinol. 298(1-2):76-83.

> 95% by SDS-PAGE

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

SDS-PAGE, Enzyme Activity

Human

Metabolism

36.7 kDa (323aa)

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY

Non-Tagged

E.coli

Recombinant Protein

Q04828

Aldo-keto Reductase1C1, AKR1C1, 2-ALPHA-HSD, 20-ALPHA-HSD, C9, DD1, DD1/DD2, DDH, DDH1, H-37, HAKRC, MBAB, MGC8954,ATGP3275-10ug,ATGP3275-20ug,ATGP3275-50ug,ATGP3275-100ug,ATGP3275-250ug,ATGP3275-500ug,ATGP3275-1mg,ATGP3275-10,ATGP3275-20,ATGP3275-50,ATGP3275-100,ATGP3275-250,ATGP3275-500,ATGP3275-1