product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant human Alkaline Phosphatase/ALPP protein
catalog :
ATGP3266
quantity :
10 ug
price :
105 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant human Alkaline Phosphatase/ALPP protein
Domain :
23-506aa
Catalog number (SKU) :
ATGP3266
NCBI :
AAH09647.1
Description :
ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Alternative Names :
ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1
Concentration :
0.5mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Kozlenkov A., et al. (2002) J Biol Chem. 277(25):22992-22999.;; Naka Stec B., et al. (2010) Acta Crystallogr Sect F Struct Biol Cryst Commun. 66(8):866-870.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 2,500unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Applications :
SDS-PAGE, Enzyme Activity
Antigen Species :
Human
Gene Category :
Enzymes & Proteases
Molecular Weight :
53.9 kDa (494aa)
Endotoxin :
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment and Pics :
Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
Amino Acid Sequence :
IIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTD
Tag :
His-Tag
Expression System :
Baculovirus
Product Type Name :
Recombinant Protein
UniProt No. :
P05187
Internal Search Terms :
Alkaline Phosphatase, ALPP, ALPI, ALPP, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, PLAP-1,ATGP3266-10ug,ATGP3266-20ug,ATGP3266-50ug,ATGP3266-100ug,ATGP3266-250ug,ATGP3266-500ug,ATGP3266-1mg,ATGP3266-10,ATGP3266-20,ATGP3266-50,ATGP3266-100,ATGP3266-250,ATGP3266-500,ATGP3266-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.