Recombinant human Cystatin B protein | NKMAX ATGP3262 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Cystatin B protein

catalog :

ATGP3262

quantity :

10 ug

price :

120 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Cystatin B protein

Domain :

1-98aa

Catalog number (SKU) :

ATGP3262

NCBI :

NP_000091

Description :

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Cystatin-B, Stefin B, PME, CST6

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 50mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Turk V, et al (1991) FEBS Lett. 285 (2): 213-9. ;; Jarvinen M, et al, (1988) Acta Histochem. 82 (1): 5-18.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

13 kDa (118aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

The IC50 value is < 3.0nM. The inhibitory function of Cystatin B on protease activity of papain was measured by a fluorometric assay using Z-FR-AMC at pH 7.5 at 25C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P04080

Internal Search Terms :

Cystatin B, Cystatin-B, Stefin B, PME, CST6,ATGP3262-10ug,ATGP3262-20ug,ATGP3262-50ug,ATGP3262-100ug,ATGP3262-250ug,ATGP3262-500ug,ATGP3262-1mg,ATGP3262-10,ATGP3262-20,ATGP3262-50,ATGP3262-100,ATGP3262-250,ATGP3262-500,ATGP3262-1

more info or order :

NKMAX product webpage