Recombinant human NME4 protein | NKMAX ATGP3254 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human NME4 protein

catalog :

ATGP3254

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human NME4 protein

Domain :

33-187aa

Catalog number (SKU) :

ATGP3254

NCBI :

NP_005000

Description :

NME4, also known as nucleoside diphosphate kinase, mitochondrial, belongs to the NDK family. NME4 are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates. The enzymes are products of the nm23 gene family, which includes NME4. NME4 plays a major role in the synthesis of nucleoside triphosphates other than ATP. Recombinant human NME4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D

Concentration :

0.5mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Milon L., et al. (1997) Hum. Genet. 99:550-557;; Milon L., et al. (2000) Biol. Chem. 275:14264-14272

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 120unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

19.6 kDa (176aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

Amino Acid Sequence :

PSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

O00746

Internal Search Terms :

NME4, Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D,ATGP3254-10ug,ATGP3254-20ug,ATGP3254-50ug,ATGP3254-100ug,ATGP3254-250ug,ATGP3254-500ug,ATGP3254-1mg,ATGP3254-10,ATGP3254-20,ATGP3254-50,ATGP3254-100,ATGP3254-250,ATGP3254-500,ATGP3254-1

more info or order :

NKMAX product webpage