protein

Recombinant human NM23-H1/NME1 protein

ATGP3250

10 ug

66 USD

Recombinant human NM23-H1/NME1 protein

1-152aa

ATGP3250

NP_000260.1

Non-metastatic cells 1 (NME1), also known as NM23-H1, originally identified as a candidate metastasis suppressor gene. NME1 is expressed in different tumor types where their levels have been alternatively associated with reduced or increased metastatic potential. Reductions in NME1 expression have been significantly associated with aggressive behavior in melanoma, breast, colon, and gastric carcinomas. On the contrary, high levels of NME1 gene expression are noted in the advanced stage of thyroid carcinomas. Recombinant human NME1 was expressed in E. coli and purified by using conventional chromatography techniques.

NME/NM23 nucleoside diphosphate kinase 1, Nucleoside diphosphate kinase A, Granzyme A-activated Dnase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NDK A, NDP kinase A, NDPKA, NM23

1mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Tee YT., et al. (2006) Taiwan J Obstet Gynecol. 45(2):107-13.;; Negroni A., et al. (2000) Cell Death Differ. 7(9):843-50.

> 90% by SDS-PAGE

Specific activity is > 1,200unit/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

SDS-PAGE, Enzyme Activity

Human

Cancer

17.1 kDa (152aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 1,200 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.

MANCERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVGLKF MQASEDLLKE HYVDLKDRPF FAGLVKYMHS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVESAEKEI GLWFHPEELV DYTSCAQNWI YE

Non-Tagged

E.coli

Recombinant Protein

P15531

NME1, Non-metastatic cells 1, Nucleoside diphosphate kinase A, NDP kinase A, AWD, GAAD, NB, NBS, NDPK-A, NM23, NM23-H1,ATGP3250-10ug,ATGP3250-20ug,ATGP3250-50ug,ATGP3250-100ug,ATGP3250-250ug,ATGP3250-500ug,ATGP3250-1mg,ATGP3250-10,ATGP3250-20,ATGP3250-50,ATGP3250-100,ATGP3250-250,ATGP3250-500,ATGP3250-1, ATGP0361