Recombinant human PGK1 protein | NKMAX ATGP3249 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human PGK1 protein

catalog :

ATGP3249

quantity :

10 ug

price :

45 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human PGK1 protein

Domain :

1-417aa

Catalog number (SKU) :

ATGP3249

NCBI :

NP_000282

Description :

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1, 3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGKA, MIG10

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Svaasand EK., et al. (2007) Muscle Nerve. 36(5):679-84. ;; Valentin C., et al. (1998) Hum Mutat. 12(4):280-7.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: > 600unit/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

46.8 kDa (437aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity > 600 units/mg. One unit will convert 1 umole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00558

Internal Search Terms :

PGK1, Phosphoglycerate kinase 1, MGC117307, MGC142128, MGC8947, MIG10, PGKA,ATGP3249-10ug,ATGP3249-20ug,ATGP3249-50ug,ATGP3249-100ug,ATGP3249-250ug,ATGP3249-500ug,ATGP3249-1mg,ATGP3249-10,ATGP3249-20,ATGP3249-50,ATGP3249-100,ATGP3249-250,ATGP3249-500,ATGP3249-1

more info or order :

NKMAX product webpage