Recombinant human Carbonic Anhydrase 8/CA8 protein | NKMAX ATGP3242 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Carbonic Anhydrase 8/CA8 protein

catalog :

ATGP3242

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Carbonic Anhydrase 8/CA8 protein

Domain :

1-290aa

Catalog number (SKU) :

ATGP3242

NCBI :

NP_004047

Description :

CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, this protein lacks carbonic anhydrase activity (i. e., the reversible hydration of carbon dioxide). It continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). Recombinant human CA8 protein fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Alternative Names :

Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP, CA8

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Turkmen S., et al. (2009) PLoS Genet. 5:E1000487-E1000487.;; Skaggs L.A., et al. (1993) Gene. 126:291-292.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 450pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Cancer

Molecular Weight :

35.5 kDa (314aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Bioactivity Comment and Pics :

Specific activity is > 450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MADLSF IEDTVAFPEK EEDEEEEEEG VEWGYEEGVE WGLVFPDANG EYQSPINLNS REARYDPSLL DVRLSPNYVV CRDCEVTNDG HTIQVILKSK SVLSGGPLPQ GHEFELYEVR FHWGRENQRG SEHTVNFKAF PMELHLIHWN STLFGSIDEA VGKPHGIAII ALFVQIGKEH VGLKAVTEIL QDIQYKGKSK TIPCFNPNTL LPDPLLRDYW VYEGSLTIPP CSEGVTWILF RYPLTISQLQ IEEFRRLRTH VKGAELVEGC DGILGDNFRP TQPLSDRVIR AAFQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P35219

Internal Search Terms :

Carbonic Anhydrase VIII, Carbonic anhydrase-related protein, CA-VIII, CALS, CAMRQ3, CARP,ATGP3242-10ug,ATGP3242-20ug,ATGP3242-50ug,ATGP3242-100ug,ATGP3242-250ug,ATGP3242-500ug,ATGP3242-1mg,ATGP3242-10,ATGP3242-20,ATGP3242-50,ATGP3242-100,ATGP3242-250,ATGP3242-500,ATGP3242-1

more info or order :

NKMAX product webpage