Recombinant human Carbonic Anhydrase 1/CA1 protein | NKMAX ATGP3241 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Carbonic Anhydrase 1/CA1 protein

catalog :

ATGP3241

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Carbonic Anhydrase 1/CA1 protein

Domain :

1-261aa

Catalog number (SKU) :

ATGP3241

NCBI :

NP_001122301

Description :

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E. coli and purified by conventional chromatography techniques.

Alternative Names :

Carbonic anhydrase I, CA-I, Carbonate dehydratase I, Carbonic anhydrase B, CAB, Cyanamide hydratase CA1, Car1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Nogradi A., et al. (1998). Am J Pathol. 153:1-4.;; Ferry JG., et al. (1999). Proc Natl Acad Sci U S A. 96(26):15184-9.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 300pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

31.0 kDa (281aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C.

Amino Acid Sequence :

MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P00915

Internal Search Terms :

Carbonic Anhydrase I, CA1, CA-I, CAB,ATGP3241-10ug,ATGP3241-20ug,ATGP3241-50ug,ATGP3241-100ug,ATGP3241-250ug,ATGP3241-500ug,ATGP3241-1mg,ATGP3241-10,ATGP3241-20,ATGP3241-50,ATGP3241-100,ATGP3241-250,ATGP3241-500,ATGP3241-1

more info or order :

NKMAX product webpage