Recombinant E.coli D-lactate dehydrogenase/LDHA protein | NKMAX ATGP3236 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant E.coli D-lactate dehydrogenase/LDHA protein

catalog :

ATGP3236

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant E.coli D-lactate dehydrogenase/LDHA protein

Domain :

1-329aa

Catalog number (SKU) :

ATGP3236

NCBI :

NP_415898.1

Description :

Idha, also known as D-lactate dehydrogenase, belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. In enzymology, a D-lactate dehydrogenase (cytochrome) is an enzyme that catalyzes the chemical reaction. Thus, the two substrates of this enzyme are (D) -lactate and ferricytochrome c, whereas its two products are pyruvate and ferrocytochrome c. Recombinant E. coli IdhA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

NAD-dependent, JW1375, htpH, hslI, hslF, Fermentative D-lactate dehydrogenase, ECK1377

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 100mM NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Atlante, A., et al. (2005) Biochim. Biophys. Acta 1708 (1): 13-22.;; Martin Engqvist, et al. (2009) J Biol Chem. 284 (September 11): 25026-25037.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 200unit/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

E.coli

Gene Category :

Redox Proteins

Molecular Weight :

39.1 kDa (353aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.

Amino Acid Sequence :

MKLAVY STKQYDKKYL QQVNESFGFE LEFFDFLLTE KTAKTANGCE AVCIFVNDDG SRPVLEELKK HGVKYIALRC AGFNNVDLDA AKELGLKVVR VPAYDPEAVA EHAIGMMMTL NRRIHRAYQR TRDANFSLEG LTGFTMYGKT AGVIGTGKIG VAMLRILKGF GMRLLAFDPY PSAAALELGV EYVDLPTLFS ESDVISLHCP LTPENYHLLN EAAFEQMKNG VMIVNTSRGA LIDSQAAIEA LKNQKIGSLG MDVYENERDL FFEDKSNDVI QDDVFRRLSA CHNVLFTGHQ AFLTAEALTS ISQTTLQNLS NLEKGETCPN ELV

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P52643

Internal Search Terms :

D-lactate dehydrogenase, ldhA, fermentative D-lactate dehydrogenase NAD-dependent, fermentative D-lactate dehydrogenase, NAD-dependent, ECK1377, hslF, hslI, htpH, JW1375,ATGP3236-10ug,ATGP3236-20ug,ATGP3236-50ug,ATGP3236-100ug,ATGP3236-250ug,ATGP3236-500ug,ATGP3236-1mg,ATGP3236-10,ATGP3236-20,ATGP3236-50,ATGP3236-100,ATGP3236-250,ATGP3236-500,ATGP3236-1

more info or order :

NKMAX product webpage