Recombinant human FBPase 1 protein | NKMAX ATGP3234 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human FBPase 1 protein

catalog :

ATGP3234

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human FBPase 1 protein

Domain :

1-338aa

Catalog number (SKU) :

ATGP3234

NCBI :

NP_000498.2

Description :

Fructose-1, 6-bisphosphatase 1 (FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1, 6-bisphosphate to fructose 6-phosphate and inorganic phosphate. Fructose-1, 6-diphosphatase deficiency is associated with hypoglycemia and metabolic acidosis. Recombinant FBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

FBPase1, FBP, FBP1

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Rabenhorst U., et al. (2009) Hepatology. 50(4):1121-9. ;; Hirota K., et al. (2008) Nature. 456(7218):130-4.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 7,000pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Metabolism

Molecular Weight :

39.0 kDa (358aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 7,000 pmol/min/ug obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 pmole of fructose 1,6 diphosphate to fructose 6-phosphate and inorganic phosphate per minute at pH 9.5 at 37C.

Amino Acid Sequence :

MADQAPFDTD VNTLTRFVME EGRKARGTGE LTQLLNSLCT AVKAISSAVR KAGIAHLYGI AGSTNVTGDQ VKKLDVLSND LVMNMLKSSF ATCVLVSEED KHAIIVEPEK RGKYVVCFDP LDGSSNIDCL VSVGTIFGIY RKKSTDEPSE KDALQPGRNL VAAGYALYGS ATMLVLAMDC GVNCFMLDPA IGEFILVDKD VKIKKKGKIY SLNEGYARDF DPAVTEYIQR KKFPPDNSAP YGARYVGSMV ADVHRTLVYG GIFLYPANKK SPNGKLRLLY ECNPMAYVME KAGGMATTGK EAVLDVIPTD IHQRAPVILG SPDDVLEFLK VYEKHSAQ

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

P09467

Internal Search Terms :

FBPase1, FBPase1, FBP, FBP1,ATGP3234-10ug,ATGP3234-20ug,ATGP3234-50ug,ATGP3234-100ug,ATGP3234-250ug,ATGP3234-500ug,ATGP3234-1mg,ATGP3234-10,ATGP3234-20,ATGP3234-50,ATGP3234-100,ATGP3234-250,ATGP3234-500,ATGP3234-1

more info or order :

NKMAX product webpage