protein

Recombinant human GSK-3 beta protein

ATGP3221

10 ug

80 USD

Recombinant human GSK-3 beta protein

1-420aa

ATGP3221

NP_001139628.1

GSK3B, also known as glycogen synthase kinase-3 beta, acts as a negative regulator in the hormonal control of glucose homeostasis. Also, this protein has diverse functions as Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Recombinant human GSK3B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

GSK-3 beta, Serine/threonine-protein kinase GSK3B

0.25mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 0.5mM PMSFand 30% glycerol

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Desai SS., et al. (2014) J. Biol. Chem. 289(9):5386-5398.;; Cheng DD., et al. (2014) Biochem. Biophys. Res. Commun. 443(2):598-603.

> 90% by SDS-PAGE

SDS-PAGE

Human

Neuroscience

47.5 kDa (426aa)

< 1 EU per 1ug of protein (determined by LAL method)

MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKVFRPRTP PEAIALCSRL LEYTPTARLT PLEACAHSFF DELRDPNVKL PNGRDTPALF NFTTQELSSN PPLATILIPP HARIQAAAST PTNATAASDA NTGDRGQTNN AASASASNST

His-Tag

Baculovirus

Recombinant Protein

P49841

GSK3 b, GSK-3 beta, Serine/threonine-protein kinase GSK3B,ATGP3221-10ug,ATGP3221-20ug,ATGP3221-50ug,ATGP3221-100ug,ATGP3221-250ug,ATGP3221-500ug,ATGP3221-1mg,ATGP3221-10,ATGP3221-20,ATGP3221-50,ATGP3221-100,ATGP3221-250,ATGP3221-500,ATGP3221-1

Jung SH, et al. Identification of transglutaminase 2 kinase substrates using a novel on-chip activity assay. (Biosens Bioelectron. 2016){https://pubmed.ncbi.nlm.nih.gov/27040940/};;Jung SH, et al. Systematic investigation of protein kinase A substrate proteins using on-chip protein kinase kinetic profiling. (Analyst. 2017){https://pubmed.ncbi.nlm.nih.gov/28536714/}