product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant rat CNTF protein
catalog :
ATGP3219
quantity :
10 ug
price :
105 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant rat CNTF protein
Domain :
1-200aa
Catalog number (SKU) :
ATGP3219
NCBI :
NP_037298
Description :
CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration :
0.5mg/ml (determined by Bradford assay)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Linker R., et al. (2009) Crit Rev Immunol. 29(1):43-68.;; Stahl N., et al. (1994) J Neurobiol. 25(11):1454-66.
Purity :
> 95% by SDS-PAGE
Bioactivity Comment Only :
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤40ng/ml.
Applications :
SDS-PAGE,Bioactivity
Antigen Species :
Rat
Gene Category :
Neuroscience
Molecular Weight :
25.2 kDa (223aa) confirmed by MALDI-TOF
Endotoxin :
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment and Pics :
Rat CNTF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤40 ng/ml.
Amino Acid Sequence :
MAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P20294
Internal Search Terms :
CNTF,ATGP3219-10ug,ATGP3219-20ug,ATGP3219-50ug,ATGP3219-100ug,ATGP3219-250ug,ATGP3219-500ug,ATGP3219-1mg,ATGP3219-10,ATGP3219-20,ATGP3219-50,ATGP3219-100,ATGP3219-250,ATGP3219-500,ATGP3219-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.