Recombinant human NMNAT-1 protein | NKMAX ATGP3194 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human NMNAT-1 protein

catalog :

ATGP3194

quantity :

10 ug

price :

57 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human NMNAT-1 protein

Domain :

1-279aa

Catalog number (SKU) :

ATGP3194

NCBI :

NP_073624

Description :

NMNAT1, also known as NMNAT or PNAT1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) or nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant human NMNAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, NMN/NaMN adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, PNAT1, NMNAT

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT 1mM EDTA

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Emanuelli M. et al. (2001) J Biol Chem. 276(1):406-12.;; Zhou T,.,et al. (2002) J Biol Chem. 277(15):13148-54.

Purity :

> 95% by SDS-PAGE

Bioactivity Comment Only :

Specific activity is > 7,000pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

Applications :

SDS-PAGE, Enzyme Activity

Antigen Species :

Human

Gene Category :

Neuroscience

Molecular Weight :

36.0 kDa (315aa) confirmed by MALDI-TOF

Bioactivity Comment and Pics :

Specific activity is > 7,000 pmol/min/ug, and was obtained by measuring the beta-NAD from nicotinamide mononucleotide and ATP per minute at pH 8.0 at 37C.

Amino Acid Sequence :

MENS EKTEVVLLAC GSFNPITNMH LRLFELAKDY MNGTGRYTVV KGIISPVGDA YKKKGLIPAY HRVIMAELAT KNSKWVEVDT WESLQKEWKE TLKVLRHHQE KLEASDCDHQ QNSPTLERPG RKRKWTETQD SSQKKSLEPK TKAVPKVKLL CGADLLESFA VPNLWKSEDI TQIVANYGLI CVTRAGNDAQ KFIYESDVLW KHRSNIHVVN EWIANDISST KIRRALRRGQ SIRYLVPDLV QEYIEKHNLY SSESEDRNAG VILAPLQRNT AEAK

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q9HAN9

Internal Search Terms :

PNAT1, PNAT 1, NMNAT1, NMNAT 1, NMNAT, NMN/NaMN adenylyltransferase1, NMN/NaMN adenylyltransferase 1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase1, Nicotinamide nucleotide adenylyltransferase 1, Nicotinamide nucleotide adenylyltransferase, Leber's congenital amaurosis 9, LCA9, ATGP3194-10ug, ATGP3194-20ug, ATGP3194-50ug, ATGP3194-100ug, ATGP3194-250ug, ATGP3194-500ug, ATGP3194-1mg, ATGP3194-010, ATGP3194-020, ATGP3194-050, ATGP3194-100, ATGP3194-250, ATGP3194-500, ATGP3194-01M

more info or order :

NKMAX product webpage