protein

Recombinant mouse TNF-alpha protein

ATGP3184

10 ug

140 USD

Recombinant mouse TNF-alpha protein

80-235aa

ATGP3184

NP_038721

TNF, also known as tumor necrosis factor isoform 1, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Mouse TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

1mg/ml (determined by absorbance at 280nm)

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Marafini I., et al. (2015) PLoS One. 10(5):e0126291.;; Zelova H., et al. (2013) Inflamm Res. 62(7):641-651.

> 90% by SDS-PAGE

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.02ng/ml.

SDS-PAGE, Bioactivity

Mouse

Hormones & Cytokines

18kDa (162aa)

< 1 EU per 1ug of protein (determined by LAL method)

Mouse TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

LRSSSQNSSD KPVAHVVANH QVEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLVYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV NLLSAVKSPC PKDTPEGAEL KPWYEPIYLG GVFQLEKGDQ LSAEVNLPKY LDFAESGQVY FGVIALHHHH HH

His-Tag

Baculovirus

Recombinant Protein

P06804

TNF-a, Tnf, DIF, TNF-a, TNF-alpha, Tnfa, TNFalpha, Tnfsf1a, TNFSF2, ATGP3184-10ug,ATGP3184-20ug,ATGP3184-50ug,ATGP3184-100ug,ATGP3184-250ug,ATGP3184-500ug,ATGP3184-1mg, ATGP3184-10,ATGP3184-20,ATGP3184-50,ATGP3184-100,ATGP3184-250,ATGP3184-500,ATGP3184-1,ATGP3184-10ug,ATGP3184-20ug,ATGP3184-50ug,ATGP3184-100ug,ATGP3184-250ug,ATGP3184-500ug,ATGP3184-1mg,ATGP3184-10,ATGP3184-20,ATGP3184-50,ATGP3184-100,ATGP3184-250,ATGP3184-500,ATGP3184-1

Kim MJ, et al. Paeonia lactiflora Root Extract and Its Components Reduce Biomarkers of Early Atherosclerosis via Anti-Inflammatory and Antioxidant Effects In Vitro and In Vivo. (Antioxidants (Basel). 2021){https://pubmed.ncbi.nlm.nih.gov/34679642/}