protein

Recombinant human AST1/GOT1 protein

ATGP3177

4 ug

30 USD

Recombinant human AST1/GOT1 protein

1-413aa

ATGP3177

NP_002070

Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Aspartate transaminase 1, AST1, SGOT, AST, cAspAT, Cysteine aminotransferase cytoplasmic, Cysteine transaminase, Cytoplasmic, cCAT, Transaminase A

0.5mg/ml (determined by Bradford assay)

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22): 5293-9.;; Totan A., et al. (2006) Clin Chem Lab Med. 44(5):612-5.

> 95% by SDS-PAGE

Specific activity is > 50unit/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

SDS-PAGE, Enzyme Activity

Human

Metabolism

48.4 kDa (433aa) confirmed by MALDI-TOF

< 1 EU per 1ug of protein (determined by LAL method)

Specific activity is > 50 units/mg, and is defined as the amount of enzyme that converts 1umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25C.

MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SVMKHRFLFP FFDSAYQGFA SGNLERDAWA IRYFVSEGFE FFCAQSFSKN FGLYNERVGN LTVVGKEPES ILQVLSQMEK IVRITWSNPP AQGARIVAST LSNPELFEEW TGNVKTMADR ILTMRSELRA RLEALKTPGT WNHITDQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINV SGLTTKNLDY VATSIHEAVT KIQ

His-Tag

E.coli

Recombinant Protein

P17174

GOT1, Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18,ATGP3177-10ug,ATGP3177-20ug,ATGP3177-50ug,ATGP3177-100ug,ATGP3177-250ug,ATGP3177-500ug,ATGP3177-1mg,ATGP3177-10,ATGP3177-20,ATGP3177-50,ATGP3177-100,ATGP3177-250,ATGP3177-500,ATGP3177-1

Yoshida T, et al. A covalent small molecule inhibitor of glutamate-oxaloacetate transaminase 1 impairs pancreatic cancer growth. (Biochem Biophys Res Commun. 2020){https://pubmed.ncbi.nlm.nih.gov/31787239/}