product summary
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company name :
NKMAX
product type :
protein
product name :
Recombinant rat ALT1/GPT1 protein
catalog :
ATGP3161
quantity :
20 ug
price :
80 USD
more info or order :
product information
Product Type :
Proteins
Item Name :
Recombinant rat ALT1/GPT1 protein
Domain :
1-496aa
Catalog number (SKU) :
ATGP3161
NCBI :
NP_112301
Description :
Gpt also known as Alanine aminotransferase 1, is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate. The Gpt is widely distributed in various tissues from animals and even in some kind of plants. It is suggested that c-ALT is associated to the utilization of pyruvate in glycolysis and m-ALT is involved in the conversion of alanine to pyruvate for gluconeogenesis. Recombinant rat Gpt, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques
Alternative Names :
Alanine aminotransferase 1
Concentration :
1mg/ml (determined by absorbance at 280nm)
Formulation :
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Storage :
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
General References :
Yang RZ., et al. (2009) Hepatology. 49(2):598-607.;;Ishiguro M., et al. (1991) Biochemistry. 30(43):10451-7.
Purity :
> 90% by SDS-PAGE
Bioactivity Comment Only :
Specific activity is > 60unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.
Applications :
Enzyme Activity,SDS-PAGE
Antigen Species :
Rat
Gene Category :
Metabolism
Molecular Weight :
57.5 kDa (519aa)
Bioactivity Comment and Pics :
Specific activity is > 60units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C.
Amino Acid Sequence :
MGSSHHHHHH SSGLVPRGSH MGSMASRVND QSQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVSGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFKEGL FLMADEVYQD NVYAEGSQFH SFKKVLMEMG PPYSTQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMGKLMSVR LCPPVPGQAL MDMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ VQLPLKAVQR AQELGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RLLLEKLSHF HAKFTHEYS
Tag :
His-Tag
Expression System :
E.coli
Product Type Name :
Recombinant Protein
UniProt No. :
P25409
Internal Search Terms :
ALT1, GPT1, Alanine aminotransferase 1,ATGP3161-10ug,ATGP3161-20ug,ATGP3161-50ug,ATGP3161-100ug,ATGP3161-250ug,ATGP3161-500ug,ATGP3161-1mg,ATGP3161-10,ATGP3161-20,ATGP3161-50,ATGP3161-100,ATGP3161-250,ATGP3161-500,ATGP3161-1
more info or order :
company information
NKMAX
SNUH Health Care Innovation Park 6F
172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
https://www.nkmaxbio.com172 Dolma-ro, Bundang-gu
Seongnam-si, Gyeonggi-do
82-31-8017-8114
headquarters: South Korea
NKMAX Co. Ltd. is a biotech company that manufactures and supplies a unique line of quality proteins and antibodies worldwide to both researchers and retailers.