Recombinant human TNF-alpha protein | NKMAX ATGP3152 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human TNF-alpha protein

catalog :

ATGP3152

quantity :

10 ug

price :

80 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human TNF-alpha protein

Domain :

77-233aa

Catalog number (SKU) :

ATGP3152

NCBI :

NP_000585.2

Description :

TNF, also known as tumor necrosis factor, is cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, under certain conditions it can stimulate cell proliferation and induce cell differentiation. Recombinant human TNF, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alternative Names :

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Concentration :

1mg/ml (determined by absorbance at 280nm)

Formulation :

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Friedmann E. et al., (2006) Nat. Cell Biol. 8:843-848.;; Zhang XM. et al., (1992) J. Biol. Chem. 267:24069-24075.

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 0.2ng/ml.

Applications :

SDS-PAGE, Bioactivity

Antigen Species :

Human

Gene Category :

Immunology

Molecular Weight :

18.1 kDa (163aa)

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Human TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Amino Acid Sequence :

VRSSSRT PSDKPVAHVV ANPQAEGQLQ WLNRRANALL ANGVELRDNQ LVVPSEGLYL IYSQVLFKGQ GCPSTHVLLT HTISRIAVSY QTKVNLLSAI KSPCQRETPE GAEAKPWYEP IYLGGVFQLE KGDRLSAEIN RPDYLDFAES GQVYFGIIAL

Tag :

His-Tag

Expression System :

Baculovirus

Product Type Name :

Recombinant Protein

UniProt No. :

P01375

Internal Search Terms :

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFA, TNFSF2,ATGP3152-10ug,ATGP3152-20ug,ATGP3152-50ug,ATGP3152-100ug,ATGP3152-250ug,ATGP3152-500ug,ATGP3152-1mg,ATGP3152-10,ATGP3152-20,ATGP3152-50,ATGP3152-100,ATGP3152-250,ATGP3152-500,ATGP3152-1

more info or order :

NKMAX product webpage