Recombinant human Glyoxalase I protein | NKMAX ATGP3144 product information

product summary

company name :

NKMAX

product type :

protein

product name :

Recombinant human Glyoxalase I protein

catalog :

ATGP3144

quantity :

4 ug

price :

30 USD

more info or order :

NKMAX product webpage

product information

Product Type :

Proteins

Item Name :

Recombinant human Glyoxalase I protein

Domain :

1-184aa

Catalog number (SKU) :

ATGP3144

NCBI :

NP_006699

Description :

Lactoylglutathione lyase, also known as GLO1, belongs to the glyoxalaseIfamily. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione. This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alternative Names :

Lactoylglutathione lyase, Glyoxalase I, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase

Concentration :

1mg/ml (determined by Bradford assay)

Formulation :

Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Storage :

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

General References :

Ridderstrom M., et al. (1996) Biochem J 314(Pt2) 463-7;; Sakamoto H., et al. (2000) Blood 95(10): 3214-8

Purity :

> 90% by SDS-PAGE

Bioactivity Comment Only :

Specific activity: > 400unit/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.

Applications :

Enzyme Activity,SDS-PAGE

Antigen Species :

Human

Gene Category :

Enzymes & Proteases

Molecular Weight :

20.7 kDa (184aa) confirmed by MALDI-TOF

Endotoxin :

< 1 EU per 1ug of protein (determined by LAL method)

Bioactivity Comment and Pics :

Specific activity > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathione from methylglyoxal and reduced glutathione per minute at pH6.5 at 25C.

Amino Acid Sequence :

ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH

Tag :

His-Tag

Expression System :

E.coli

Product Type Name :

Recombinant Protein

UniProt No. :

Q04760

Internal Search Terms :

Glyoxalase I, Lactoylglutathione lyase, Glyoxalase I, Glx 1, GLO-1 Methylglyoxalase, Aldoketomutase,ATGP3144-10ug,ATGP3144-20ug,ATGP3144-50ug,ATGP3144-100ug,ATGP3144-250ug,ATGP3144-500ug,ATGP3144-1mg,ATGP3144-10,ATGP3144-20,ATGP3144-50,ATGP3144-100,ATGP3144-250,ATGP3144-500,ATGP3144-1

more info or order :

NKMAX product webpage